MicroRNA-21 regulates expression of reversion inducing cysteine rich protein with Kazal motifs in MCF-7 cells
ZHENG Zhiwei
ZHAO Shulin
YANG Yuxian
ZHU Huide
ZHU Zhiwei
CHEN Lei
Abstract：Objective To explore the effects of microRNA-21 ( miR-21) on the expression of reversion inducing cysteine rich protein with Kazal motifs ( RECK ) in MCF-7 cells. Methods MCF-7 cells were cultured in vitro and transfected with miR-21 mimics and miR-21 inhibitor by ca-tionic-mediated transfected. The total RNA and protein were extracted from the culture cells after transfected forty-eight hours. The expression of miR-21 and RECK levels were detected by fluores-cent quantitative PCR and Western blotting. Results The miR-21 expression was significantly changed after forty-eight hours transfected ( P<0. 001 ) , miR-21 expression decreased significantly in miR-21 inhibitor group while miR-21 expression increased significantly in miR-21 mimics group. The RECK was significantly changed after transfected ( P<0. 001) , RECK expression increased sig-nificantly in miR-21 inhibitor group ( P<0. 001 ) while RECK expression decreased significantly in miR-21 mimics group ( P<0. 001 ) . Conclusion miR-21 can inhibit the expression of RECK in breast cancer cells, and it may be a new target to judge the prognosis of breast cancer patients and the new target of tumor therapy.
Keywords：Breast cancerMicroRNA-21 ( miR-21)Reversion inducing cysteine rich pro-tein with Kazal motifs ( RECK)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 198-201,234 )