Neuroprotective effects of troxerutin and cerebroprotein against neuronal damage induced by cerebral ischemia
LI Ruolin
LI Yanfang
LIU Fang
YANG Wen
LU Ranran
ZHONG Shanshan
ZHU Zhe
WANG Xiaoqian
YU Yingxin
REN Ming
QI Xiaokun
Abstract：Objective To investigate the neuroprotective effects of troxerutin and cerebropro-tein against neuronal damage induced by cerebral ischmia injury. Methods Male SD rats were di-vided into 3 groups ( n=10):normal control group ( NC) ,middle cerebral artery occlusion reperfu-sion (MCAO/R) group, MCAO/R+(troxerutin and cerebroprotein, TC) group (MCAO/R+TC). One hour after ischemia and 23 hours after reperfusion, 6 rats in each group were evaluated by neu-ral function test and magnetic resonance imaging ( MRI ) , and then sacrificed for determining the cerebral infarction volume with 2,3,5-triphenyltetrazolium chloride ( TTC) staining;the remaining 4 rats in each group were sacrificed, and the protein levels of Caspase 1, Caspase 3,Caspase 8 in the ischemia penumbra were determined by Western Blotting. Results After MCAO/R, cerebral infarc-tion and neurological deficit were manifested. Troxerutin and cerebroprotein was proved to be effec-tive in improving neurological functions and decreasing infarction on MCAO/R animals. Compared with MCAO/R groups, the expression of Caspase 1, Caspase 3, Caspase 8 in NC groups were de-creased significantly ( P<0. 05 ) . Conclusion Troxerutin and cerebroprotein has protective effect against injury induced by cerebral ischemia reperfusion, which is related to their role in inhibiting the neuronal apoptosis.
Keywords：Cerebral ischemiaTroxerutin and cerebroproteinCell apoptosis235-triphe-nyltetrazolium chloride
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 9-13 )