Ketogenic diet may improve ischemic tolerance of middle cerebral artery occlusion mice through up-regulation of hypoxia inducible factor-1 target genes
GUO Min
CUI Mei
DONG Qiang
Abstract：Objective To explore the effects of ketogenic diet on middle cerebral artery occ-lusion ( MCAO ) mice and the role of hypoxia inducible factor-1 ( HIF-1 ) target genes in this process. Methods C57BL/6 mice were randomly allocated into four groups: standard diet group, high carbohydrate diet group, ketogenic diet group and control group. Mice of the 3 intervention groups were fed with corresponding diets for 3 weeks and were afterwards made into MCAO mice models. Control group was fed with standard diet for 3 weeks without MCAO treatment. Stroke vo-lume was measured by 2,3,5-triphenyl tetrazolium chloride ( TTC) staining. Neurological function was accessed by Longa scoring method. Polymerase chain reaction ( PCR) was used to detect mRNA expression quantity of HIF-1 downstream target genes including erythropoietin ( EPO) , vascular en-dothelial growth factor ( VEGF ) , glucose transport 1 ( GLUT1 ) and monocarboxylic transporter 4 ( MCT4) in the ischemic region. Results Compared with the other 3 groups, ketogenic diet group showed smaller stroke volume, better neurological score and higher mRNA expression quantity of HIT-1 downstream target genes. Conclusion Ketogenic diet may improve brain ischemic tolerance of MCAO mice through up-regulation of HIF-1 target genes.
Keywords：Ketogenic dietMiddle cerebral artery occlusion(MCAO)Hypoxia inducible factor-1(HIF-1) target genesStroke
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-8,13 )