Protocol for cryopreservation and thawing of hematopoietic stem cells (for discussion)
SHEN Jianliang
HAN Ying
HUANG Youzhang
Abstract：The efficacy of cryopreservation of hematopoietic stem cells was influenced by a lot of factors, such as temperature, cooling rate, cell concentration, cryopreservative, thawing proce-dure, preserved period and measurement of cell functionalities.There were some differences in tech-nology and methodology between research centers in the world, so the results from their publications could be poorly compared with each other and had a weak potency in evidence-based medicine.Ex-cept for the established protocol for cryostorage of mobilized peripheral blood ( MPB) hematopoietic stem cells/hematopoietic progenitor cells ( HSC/HPC) and bone marrow ( BM) HSC/HPC in Joint Accreditation Committee of International Stem Cell Transplantation ( ISCT ) Europe and European Bone Marrow Transplantation ( EBMT) ( JACIE) guidelines, there was no guideline in China and the world.With the help of our experiences ( for many years in cryomedical field) and related litera-tures we have drawn up this protocol for cryopreservation and thawing of hematopoietic stem cells suited for petty bank in hospital.Any comments or revisions will be welcome.
Keywords：Hematopoietic stem cellsCryopreservationThawing
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 316-320 )