Method validation for RNA quality control in biobanking
RUAN Liangliang
ZHENG Peiyong
YANG Jiahong
FAN Jinli
GAN Rongxing
Abstract：Objective To perform method validation for RNA quality control .Methods There are three quality controls that are performed on isolated RNA:concentration , purity and integ-rity.The optical density ( OD) at 260 nm is used to determine the RNA concentration in a solution . The ratio of the absorbance at 260 nm to 280 nm is used to assess the RNA purity of an RNA prepa-ration.The integrity assessment is based on the RNA integrity number (RIN) system.In this work, we focused on the RNA concentration and integrity .According to the requirement of ISO/IEC 17025∶2005, the key factors of precision , accuracy and linearity testing were presented in assessing the method of RNA quantity .Precision testing on RNA integrity was also preformed .Results In this study , we focused on the validation of RNA quantitation by spectrophotometry , and performed preci-sion, accuracy and linearity assessment .Total RNA from Hela cells was used for precision testing on RNA integrity.All the data were acceptable .Conclusion The method of spectrophotometry and RIN system are qualified for RNA quality control .
Keywords：Quality management systemMethod validationPrecisionAccuracyLinearity
Publication Date：2015-05-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 161-165 )