Kinase-domain insert containing receptor gene silencing inhibited proliferation of human lung cancer cell line A549 and enhanced their sensitivity to docetaxel
ZHANG Xiuyi
Abstract：Objective This study investigated the effect of small interfering RNA-mediated kinase-domain insert containing receptor ( KDR) knock-down on proliferation of human lung cancer cell line A549 and their sensitivity to docetaxel .Methods The small interfering RNA ( siRNA) against KDR was constructed and transfected into A 549 cells with Lipofectamine TM 2000.The expre-ssion of KDR was detected by reverse transcription-polymerase chain reaction and Western Blot . Flow cytometry was used to detect the cell cycle .Sensitivity to docetaxel after transfection were exa-mined by methyl thiazolyl tetrazolium assay and clonogenic assay .Results In A549 cells, the pro-tein and mRNA levels of KDR were decreased significantly after transfection ,and reduction of proli-feration was related to an increase in the fraction of G 0/G1 phase .The sensitivity of A 549 cells to docetaxel was increased significantly after transfection .Conclusion The KDR special siRNA si-lenced KDR ,decreased A 549 cells proliferation and enhanced their sensitivity to docetaxel .
Keywords：Kinase-domain insert containing receptor(KDR)Small interfering RNA(siRNA)Lung cancerA549 cellsCell proliferationDocetaxel
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 133-136,140 )