Myofibrillogenesis regulator-1 induces hypertrophy by promoting sarcomere organization in neonatal rat cardiomyocytes
WANG Xiaoreng
LIU Xiuhua
WANG Song
LUAN Kang
Abstract：Objective Exploration of myofibrillogenesis reguiator-1 ( MR-1) by regulating the generation mechanism of causing myofibrillar hyperlrophy of the cardigmyocytes .Methods Af-ter intervention of MR-1 expressions in neonatal rat cardiomyocytes , we measured several hyper-trophic index e .g.the mRNA levels of the atrial natriuretic factor ( ANF) and brain natriuretic pep-tide ( BNP ) , the cell size , and the velocity of protein synthesis .The sarcomeric F-actin organization were detacted by staining with phalloidin-fluorescein isothiocyanate ( FITC);sublocation of myome-sin-1 and myosin regulatory light chain ( MLC-2) were assessed by semi-quantitative reverse tran-scription-polymerase chain reaction ( RT-PCR), western blot, and immunocytofluorescent assays . Results MR-1 overexpression induced cardiomyocyte hypertrophy , promoted the sarcomeric F-actin organization , increased the expression of MLC-2 and myomesin-1 ( P<0.05 ) , as well as the myome-sin-1 translocation from nucleus to cytoplasm .Those results of myomesin-1 translocation and F-actin organization were similar to the small ubiquitin modifier-1 ( SUMO-1) overexpression , MR-1 inhibi-tion by RNA interference did not induce the translocation of myomesin-1, even decrease the SUMO-1 overexpression induced myomesin-1 translocation , as well as the promoted F-actin organization . Conclusion MR-1 induces cardiomyocytes hypertrophy by promoting the MLC-2 and myomesin-1 induced myofibrillogenesis .
Keywords：Myofibrillogenesis regulator-1(MR-1)Cardiomyocyte hypertrophyF-actinMyomesin-1Myosin light chain-2(MLC-2)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 37-44 )