Application of liquid based cell production of immunohistochemical staining in the diagnosis of pleural effusion and ascites
TAN Yongxing
JIANG Weimin
LI Yanmei
TU Yunjiao
Abstract：Objective To study the liquid based cytology production technology application of immunohistochemical staining.Methods Hematoxylin and eosin staining smear samples of suspi -cious malignant tumor cells remaining preservation solution made more than LBP system and then smear for immunohistochemical stain glucose transporter -1 (Glut-1), calretinin (CR), cell surface glycoprotein MOC31, epithelial antigen (EA).Results One hundred and four cases of suspicious tumor after chest water by immune markers clearly distinguished as carcinoma or mesothelioma or reactive mesothelium cell hyperplasia .Conclusion The immunohistochemical antigen -antibody reaction is high specificity and sensitivity of biochemical reactions with specific antigen recognition . Antigen molecules in the cellular and subcellular level testing is difficult to achieve and replace , which has a broad application in cytology .Unidentified pleural effusion by cell immunohistochemical staining, combined application of CR, Glut-1, MOC31, and EA properties can clearly distinguish effusion.
Keywords：Pleural effussionSeroperitoneumCytologyThinlayerliquidbasedsmearsStaining
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 147-149 )