Study on the bioactivity of Tripterygium wilfordii extract against Culex quinquefasciatus
ZHENG Weiqing
XU Qian
LI Siping
WEI Guimei
Abstract：Objective To investigate the knockdown and adulticidal efficacy of Tripterygium wilfordii extract against Culex quinquefasciatus.Methods The bioactivity of T.wilfordii extract was evaluated according to the Laboratory Efficacy Tests and Evaluation of Public Health Insecticides for Pesticide Registration-Part 1:Spray Fluid(GB/T 13917.1-2009).Results The extract exhibited excellent knockdown effects on Cx.quinquefasciatus.Both the knockdown rate and knockdown speed increased with concentration.At a concentration of 30.00 mg/L,the 20 min knockdown rate was 21.45%.When the concentration increased by 3.375-fold(101.25 mg/L),the knockdown rate reached 77.78%,and the median knockdown time(KT50)was 9.40 min.At a concentration of 227.81 mg/L,the knockdown rate was 100%,with statistically significant concentration-dependent variations in knockdown rate(P＜0.05).The extract also demonstrated marked adulticidal activity,showing a dose-effect relationship.Post-24 h treatment,median lethal concentration(LC50)and LC90 were 58.10 mg/L and 129.80 mg/L,respectively.Among some chemical components of the extract of T.wilfordii,triptolide exhibited the most potent adulticidal activity against Cx.quinquefasciatus,achieving mortality rates of 87.78%and 100%at concentrations of 1 mg/L and 5 mg/L,respectively,with significantly higher bioactivity than the other four compounds(P＜0.05).Conclusion T.wilfordii extract exhibits potent knockdown and adulticidal effects against mosquitoes,positioning triptolide as the dominant active ingredient.
Keywords：Tripterygium wilfordii extracttriptolideknockdownadulticidal activitybioactivityCulex quinquefasciatus
Publication Date：2025-12-20
Online Publishing Date：2026-01-09(First online date of this platform, not the publication date of the document)
Pages：4( 631-634 )