Analysis of beta-cypermethrin and indoxacarb 0.6％ DP by ultra performance liquid chromatography
PENG Kaijun
XIONG Xiangui
YU Yang
JIA Huixian
CHEN Yang
YANG Wenfeng
ZENG Xiaohu
ZHANG Liang
XU Jing
Abstract：Objective To establish a method to detect the beta-cypermethrin and indoxacarb 0.6％DP by ultra performance liquid chromatography(UPLC).Methods The sample was extracted by acetone and acetonitrile ultrasonically.Quantitative analysis of the active ingredient was conducted using a Waters ACQUITY UPLC ® BEH C18 chromatographic column and variable wavelength photodiode array detection(PDA)at 230 nm and 310 nm.The mobile phase was acetonitrile-water(80/20,by vol),and the mobile speed was 0.2 ml/min.The quantitative analysis was performed using external standard calibration curve.Results The linear correlation coefficients of indoxacarb and beta-cypermethrin were both 0.999 9.The average recoveries were 100.45％-105.30％and 97.88％-100.38％,respectively.The coefficients of variation were 0.44％and 0.59％(n=6),respectively.The quantitative limits were 3.15× 10-3 mg/L and 6.44×10-3 mg/L,respectively(10S/N).Conclusion The method is an ideal analytical method with the advantages of simplicity,rapidity,high precision and accuracy,and good linear relationship.
Keywords：UPLCbeta-cypermethrinindoxacarbanalytical method
Publication Date：2025-06-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 249-252 )