Screening and proportion optimization of active components of Mikania micrantha for mosquito repellency
HU Jingwen
CHENG Qianqian
LYU Bin
LIU Hongzhen
WANG Zhenhua
DU Qin
Abstract：Objective To investigate the mosquito repellent effect of Mikania micrantha,screen its mosquito repellent active ingredients and obtain efficient mosquito repellent compositions,so as to provide a reference for development of botanical mosquito repellents.Methods Different M.micrantha crude extracts were initially screened for mosquito repellent activity by the Y-type olfactometer method,and further screened for M.micrantha mosquito repellent active ingredients by qualitative analysis of the active crude extracts by GC-MS.The active ingredients were matched and combined,and the ratio of the optimal mosquito repellent composition was predicted by using a homogeneous experimental design.Results The results showed that the volatile oil was the main mosquito repellent active component of M.micrantha,containing stilbene oxide,eucalyptol and other active ingredients,and the mosquito repellent rate reached 63.80％.The combined with the homogeneous experimental design of the screening of the optimal mosquito repellent component was:9.8％caryophyllene oxide+9.9％1,8-cineole+8.6％linalool+7.9％α-terpineol+7.8％δ-cadinene.The average mosquito repellent rate of the composition was 83.20％,and the mosquito repellent effect was good.Conclusion The volatile oil of M.micrantha has mosquito repellent activity,and it contains many mosquito repellent active ingredients,which can be prepared highly efficient mosquito repellent compositions,further developed as mosquito repellent products,and provide a new resource for botanical mosquito repellents.
Keywords：Mikania micranthamosquito repellent compositionactive componentsvolatile oiluniform design
Publication Date：2025-02-19
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 12-16,35 )