Study on the application of two detection methods in the serum of Marmota himalayana in Tongren county,Qinghai Province
ZHANG Xiaolu
LI Guanghui
MA Long
YANG Jianguo
YOU Peisong
LI Xiang
JIN Yong
LI Sheng
FENG Jianping
LI Cunxiang
Abstract：Objective To detect antibodies related to Yersinia Pestis in the serum of Marmota Himalayana in Tongren county,Qinghai Province,in order to provide reference for the epidemiological investigation of plague and the conservation mechanism of Yersinia pestis in nature.Methods 200 Marmots were collected from Tongren County,Qinghai Province in 2021,and plague indirect hemagglutination test was used to detect yersinia pestis F1 antibody in marmots.A wild-type plague phage 476 isolated from the plague focus on the Qinghai-Tibet Plateau and the plague phage used for laboratory diagnosis were used as antigen.The specific immune antibody of plague phage in Marmots serum was detected by microplate method and double AGAR plate method.Results The serum antibody of Yersinia pestis F1 in M.himalayana was negative.Two strains of plague phage were neutralized with 200 M.himalayana sera,and no specific phage immune antibody was detected by drip method.Conclusion No F1 antibody or Y.pestis bacteriophage specific antibody was found in the serum of M.himalayana in Tongren County,Qinghai Province,both of which indirectly indicated that the plague focus at the sampling site in Tongren County was in a resting state.
Keywords：Marmota himalayanaYersinia pestis bacteriophageplague antibodiesepidemiology
Publication Date：2023-12-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 539-543 )