Discussion on the method of evaluating the comparison results of inter-laboratory to vector resistance monitoring

HAN Xiao-li; WANG Xi-ming; HUANG Gang; MA Li-hua; GAO Wen

Abstract：Objective To explore the method of evaluating the results of inter-laboratory comparison of vector resistance monitoring to ensure the accuracy and validity of the data of resistance monitoring in various municipal disease control agencies.Methods In the nine laboratories where Hebei province had been able to independently carry out the resistance test,H,C,Z and D laboratories were tested for resistance testing inter-laboratory comparison (comparison of the number of indicators of a total of 25),to S laboratory as a reference laboratory.The results of interlaboratory comparisons were evaluated by the Ratio Value En and Wilcoxon Signed Rank test,and their applicability was discussed.Results The result of the inter-laboratory comparison by the Ratio Value En,and the coincidence rate was 48％.The method could be used for the comparison results of inter-laboratory resistance monitoring of Musca domestica and Culex pipiens pallens.Wilcoxon Signed Rank test was used to evaluate the results of inter-laboratory comparison,and the coincidence rate was 78％.The method could be used to examine the differences between the results of the same kind of test insects on the detection of different pesticides in the two laboratories.Conclusion The Ratio Value En is more suitable for comparison between LD50 and LC50inter-laboratory,while for KT50 comparison,due to the higher numerical value,need further study.The Wilcoxon Signed Rank test can be used as a reference when performing inter-laboratory comparisons.