Establishment and identification of Blattella germanica embryonic cell line HDCDC-BGE-Ⅰ
HOU Wei-yuan
WANG Lei
CAI Wei
ZHAO Yan
Abstract：Objective To establish and identify Blattella germanica embryonic cell line.Methods The Blattella germanica embryos developed 5 d were isolated and used to primary culture.Then primary embryonic cells were subcultured for 25 times to form a cell line.The morphology,growth characteristics,chromosome karyotype and DNA amplification fingerprinting of the established cell line were studied.The freezing regenerate experiment was also carried out.Results The established cell line was named HDCDC-BGE-Ⅰ.The HDCDC-BGE-Ⅰ cells were round with a diameter of about 8-10 μm,and the cells were able to grow adhering to the bottle wall.The HDCDC-BGE-Ⅰ cells entered the logarithmic proliferation phase on the 5th day and reached the platform period on the 8th day.The population doubling time was 124.2 h.The chromosome number of the HDCDC-BGE-Ⅰ cell was 22-24,which was diploid.The results of DNA amplification finger printing confirmed that HDCDC-BGE-Ⅰ cells derived from Blattella germanica.The HDCDC-BGE-Ⅰ cells could be preserved for a long time at-80 ℃ and regenerated successfully.Conclusion The HDCDC-BGE-Ⅰ cell line is derived from Blattella germanica with homogeneous biological characteristics and genetic stability.
Keywords：Blattella germanicaembryonic cell lineHDCDC-BGE-Ⅰ
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 155-158 )