Regulatory effect of c-Myc-dependent R-loop structure on the proliferation,invasion,and migration of glioma cells
LIN Jiahan
CHEN Caiyan
CHEN Shenbo
YE Fuyue
YANG Kun
Abstract：Objective To explore the c-Myc-dependent R-loop structure and its regulatory effect on the proliferation,invasion and migration of glioma cells.Methods DRIP-PCR was used to detect the R-loop structure of c-Myc in glioma cells(U251 and U87-MG).U251 and U87-MG were transfected into empty plasmids,c-Myc overexpression plasmids,and RNASEH1 overexpression plasmids respectively,which were used respectively to establish control group(CTRL group),RNASEH1 overexpression group(RNASEH1-OE group),c-Myc overexpression group(c-Myc-OE group),and co-transfected c-Myc and RNASEH1 overexpression group(c-Myc-OE+RNASEH1-OE group).CCK-8 assay,EdU assay,scratch assay,and Transwell assay were used to detect the proliferation,migration,and invasion levels of glioma cells.The Western blot was used to detect the expression levels of RNASEH1 and c-Myc proteins expressions in glioma cells.Results c-Myc had an R-loop structure in glioma cells(P<0.05).Overexpression of RNASEH1 could knock out the c-Myc-dependent R-loop structure,further reducing glioma cell proliferation,invasion,and migration(P<0.05).Overexpression of c-Myc could upregulate the expression of knockdown RNASEH1,thereby rescuing the c-Myc-dependent R-loop structure and enhancing the invasion and migration abilities of glioma cells(P<0.05).Conclusions c-Myc-dependent R-loop structure can promote the proliferation,invasion and migration of glioma cells.
Keywords：c-MycRNASEH1R-loopsgliomaproliferationinvasionmigration
Publication Date：2026-02-28
Online Publishing Date：2026-03-13(First online date of this platform, not the publication date of the document)
Pages：7( 16-22 )