Relationship between the expression levels of ERCC1 and ARL2 in human brain glioma and the pathological features and prognosis of patients

CHEN Hengsan; GUO Chao; WANG Hu; WEI Yi; JIN Ming; YU Xiangyang; HUANG Ming; ZHOU Dongchun; SHANG Yinwu; LU Anqing

Abstract：Objective To explore the relationship between the excision repair cross-complementation group 1(ERCC1)and ADP ribosylated factor 2(ARL2)in human glioma(BG)tissues and the pathological characteristics and prognosis of patients.Methods 122 BG cases from August 2019 to August 2022 were selected.BG tumor tissue specimens obtained during surgery were included in the BG group(n=122),and normal brain tissue specimens obtained due to craniocerebral trauma during the same period were included in the control group(n=60).The expression of ERCC1 and ARL2 in brain tissue was detected by immunohistochemical method.The correlation between the ERCC1,ARL2 and the pathological features of BG was analyzed by χ2 test.The prognostic factors of human brain glioma patients were explored by Multivariate Cox regression analysis.Results The positive expression rates of ERCC1 and ARL2 in BG group were 69.67%and 66.39%,which were more than the respective figures of 28.33%and 25.00%in the control group(P＜0.05).The positive expression rates of ERCC1 and ARL2 in poorly differentiated BG patients were higher than those in moderately differentiated/highly differentiated BG patients(P＜0.05).These 122 BG patients were followed up for 2 years after discharge,and 59 of them survived,with an overall survival rate of 48.36%(59/122).The 2-year overall survival rate of patients with medium/high differentiation,ERCC1 negative and ARL2 negative was higher than that of patients with low differentiation,ERCC1 positive and ARL2 positive respectively(P＜0.05).Low differentiation(HR=2.368,95%CI:1.442-3.888),positive ERCC1(HR=2.824,95%CI:1.634-4.879),positive ARL2(HR=3.370,95%CI:1.902-5.973)were independent risk factors for prognosis of human brain glioma patients(P＜0.05).Conclusions ERCC1 and ARL2 are highly expressed in BG tissues,and their expression increases as the degree of differentiation decreases.ERCC1 and ARL2 are also associated with postoperative survival in patients.