Predictive efficacy of serum miR-15a and miR-24 levels on postoperative recurrence in cases with functional pituitary adenoma

ZHENG Tao; CHE Yuanyuan; CHU Dong; LIU Yang; HE Shiming; HUANG Yuanzhi

Abstract：Objective To investigate the predictive efficacy of serum miR-15a and miR-24 levels on postoperative recurrence in cases with functional pituitary adenoma.Methods A total of 107 cases with functional pituitary adenoma who received surgical treatment from June 2017 to June 2022 were selected as study subjects,followed up for 2 years after surgery,and divided into a recurrence group(n=22)and a non-recurrence group(n=85).Clinical data of the subjects were collected,and the serum levels of miR-15a and miR-24 were detected by real-time fluorescence quantitative PCR on the 5th days after surgery.The predictive value of serum miR-15a and miR-24 on postoperative recurrence in patients with functional pituitary adenoma was analyzed by ROC,and the influencing factors of postoperative recurrence in cases with functional pituitary adenoma were analyzed by multivariate Logistic regression.Results The levels of serum miR-15a and miR-24 in cases with recurrence were lower than those in cases without recurrence(P＜0.05).The AUC of serum miR-15a and miR-24 in predicting postoperative recurrence in cases with functional pituitary adenoma was 0.781(95%CI:0.736-0.831)and 0.819(95%CI:0.774-0.869)respectively,and the AUC of the combined prediction was 0.906(95%CI:0.856-0.951).The single factor analysis showed that the proportion of tumor stage Ⅲ to Ⅳ and pathological type invasion in the recurrence group were higher than those in the non-recurrence group(P＜0.05).Multivariate regression analysis showed that tumor stageⅢ-Ⅳ(OR=2.307,95%CI:1.433-3.715),pathological type invasion(OR=2.140,95%CI:1.266-3.619),miR-15a≤0.52(OR=2.795,95%CI:1.820-4.294)and miR-24≤0.91(OR=2.649,95%CI:1.704-4.116)were risk factors for postoperative recurrence in cases with functional pituitary adenoma(P＜0.05).Conclusions The decrease of serum miR-15a and miR-24 is closely related to postoperative recurrence in cases with functional pituitary adenoma.Serum miR-15a and miR-24 have certain predictive value for postoperative recurrence in cases with functional pituitary adenoma,and their combined detection has better predictive efficacy.