Polyethyleneimine-mediated Bmk CT gene inhibition of VEGF,MMP-9,AQP-1 expressions in C6 glioma cells
ZHANG Yatao
HU Wentao
LAN Jinxing
CAI Ling
XU Donggang
LIU Jialin
Abstract：Objective To investigate the effects of polyethyleneimine(PEI)-mediated Buthus martensii Karsch Chlorotoxin-like Toxin(Bmk CT),and Bmk CT gene transfection on the expression levels of VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65 of C6 glioma cells.Methods pEGFP-N1 and pEGFP-N1-BmK CT were respectively combined with PEI to form two complexes which were used to transfect C6 cells.After 48 hours'test by Western bloting,VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65 were detected.Results The expression levels of VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65 in the pEGFP-N1-Bmk CT group were significantly lower than those of the pEGFP-N1 group after the transfection.Conclusions PEI/pEGFP-N1-Bmk CT may suppress tumor associated edema through inhibiting the expressions of VEGF,MMP-9,AQP-1,p-ERK,p-AKT and p-NF-κBp65.
Keywords：Bmk CTGliomaVEGFMMP-9AQP-1Pathway
Publication Date：2025-04-08
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 12-16 )