Effects and mechanism of aminoguanidine on peritumoral edema in glioma

LIN Xiangdan; FAD Di

Abstract：Objective To explore the effects of aminoguanidine(AG)on brain edema around glioma(2 mm).Methods 90 male Wistar rats were randomly divided into 5 groups with 18 rats in each group:sham operation group(A),model control group(B),model+AG 75 mg/kg group(C),model+AG 150 mg/kg group(D)and model+AG 225 mg/kg group(E).The glioma model was established by injecting 10 μl C6 cells into the right caudate nucleus of Wistar rats in group B,C,D and E,whereas group A was injected 10 μl medium according to the above method.14 days later,the model was established.Group A and B were administered corresponding dose of normal saline(ip;bid),while group C,D and E were administered corresponding dose of AG(ip;bid)for 3 days.The brain water content was measured by dry-wet weight method,and the contents of Zonula Occluden1(ZO-1),Occludin,vascular endothelial growth factor(VEGF)and aquaporin 4(AQP4)were detected by immunofluorescence and Western-blot methods.Results Compared with group B,group D significantly decreased the brain water content(P<0.05),while group C and E did not significantly decrease the brain water content(P>0.05).Compared with group B,group D significantly increased the expression of ZO-1 and Occludin protein(P<0.05),and significantly decreased the expression of VEGF and AQP4 protein in the peritumoral brain tissue(P<0.05).Conclusions AG can alleviate the brain edema caused by glioma by increasing the expression of ZO-1 and Occludin and reducing the expression of AQP4 and VEGF in peritumor brain tissue.