Expression and biological role of intercellular adhesion molecule-1 in glioblastoma

DING Hui; CHEN Shenbo; YANG Liangwang; WU Ran; YE Fuyue; YANG Kun

Abstract：Objective To investigate the prognostic value of intercellular adhesion molecule-1(ICAM1)in glioblastoma(GBM)and its relationship with immunosuppression.Methods Expression and clinical data from TCGA-GBM were used to evaluate ICAMs mRNA expression and its clinical prognostic value.The expression of ICAM1 in GBM tissues and cells was evaluated by using The Human Protein Atlas database.The correlation between ICAM1 and other mRNAs in GBM was analyzed based on the cBioPortal database,and the top 100 genes with the most significant positive correlation with ICAM1 were selected for GO and pathway analysis through the DAVID database.Transcription factors that may regulate ICAM1 expression were identified by using the JASPAR database and validated by using the GSE65363 dataset of the GEO database.The relationship between ICAM1 expression and immune cell infiltration was investigated by using the CAMOIP database.Results ICAM1 was highly expressed in GBM and was associated with prognosis.ICAM1 was positively correlated with the expression of Mesenchymal marker genes and negatively correlated with the expression of proneural marker genes.NF-κB,together with ICAM1,could initiate the promoter and regulate the expression of ICAM1.In addition,ICAM1 was involved in the immune response,and the high expression of ICAM1 promoted the polarization of macrophages.At the same time,ICAM1 was highly positively correlated with immunosuppressor genes.Conclusions ICAM1 is one of the initiating factors of PMT in GBM,and plays an important role in GBM immunosuppression,which can be used as a potential biomarker to predict the prognosis of GBM patients.