The expression and function of LncRNA FOXD2-AS1 in glioma cells

BI Yuxu; GONG Pengcheng; FU Yu; YAO Qian; NI Wei

Abstract：Objective To study on the impact of lncRNA FOXD2-AS1 on the biological behavior of glioma.Methods Firstly,the expression of FOXD2-AS1 in three glioma cell lines,namely U251,LN229,and U87,was detected by qPCR experiment.Two cell lines with high expression were screened for culturing.Subsequently,lentiviral vectors were constructed to transfect the two cell lines to knockdown FOXD2-AS1 as the experimental group.The cells transfected with empty lentiviral particles were used as the negative control group,and the cells cultured conventionally without transfection were used as the blank group.The scratch test and Transwell test were used to detect the migration and invasion ability of each group of cells in the two glioma cell lines.Transcriptome sequencing was performed after Knockdown of FOXD2-AS1,and the intersection of differential genes was taken,and enrichment analysis was performed on the common differential genes.Results The qPCR experiment showed that FOXD2-AS1 was significantly highly expressed in the two glioma cell lines LN229 and U251.Compared with the negative control group and the blank group,when FOXD2-AS1 was Knockdown by lentiviral vector transfection,the migration and invasion ability of the experimental group cells in the two glioma cell lines decreased significantly.Bioinformatics database analysis found that there were 48 related differentially expressed genes in the two glioma cell lines when FOXD2-AS1 was Knockdown.Further enrichment analysis through KEGG pathway found that the Knockdown of FOXD2-AS1 in the two glioma cell lines was correlated with multiple signaling pathways such as focal adhesion in cell processes,neuroactive ligand-receptor interaction in environmental information processing,spliceosome in genetic information processing,cancer pathways in human diseases,metabolic pathways in metabolism,complement and coagulation cascade in organic systems.Conclusion LncRNA FOXD2-AS1 plays a role in the migration and invasion of glioma cells,and its possible mechanism of action may require further study in the future.