High and specific expression of Fibronectin(FN1)in cerebral vascular cells as an abnormal expression in glioma tumor cells
MING Anhong
ZHU Mengting
LYU Rui
JI Ang
XUE Xiaoyan
ZHANG Xiang
MAO Xinggang
Abstract：Objective To investigate the expression patterns of fibronectin(FN1),an important protein in the extracellular matrix(ECM),in normal brain tissue and gliomas,with special attention to its expression in different types of cells.Methods Single-cell sequencing data were used to analyze the expression of FN1 in different types of cells in normal human and mouse brain tissues.Further analysis of single-cell sequencing data in glioblastoma(GBM)was performed.Finally,the expression of FN1 protein in normal brain and glioma tissues,especially in vascular structures,was studied by immunohistochemical staining.Results In both human and mouse normal brain single-cell sequencing data,FN1 showed consistent expression patterns and was specifically overexpressed in vascular structural cells,including endothelial cells and pericytes.In the single-cell sequencing data of GBM,FN1 was not only highly expressed in vascular endothelium and pericytes,but also in other types of cells such as tumor cells,including glioma stem cells(GSC),immune cells,etc.Further immunohistochemical staining results confirmed that FN1 protein is highly expressed in vascular structures in the normal brain,while in high-grade gliomas,it is also highly expressed in tumor cells in addition to vascular structures.Conclusion FN1 is a type of molecule that is highly expressed in vascular structure in normal brain tissue,while in GBM,it is abnormally expressed in tumor cells,stem cells,immune cells,etc.,reflecting the high heterogeneity and plasticity of GBM tumor cells.
Keywords：GlioblastomaEndothelial cellPericyteVasculatureVesselsMacrophageMicrogliaTumor stem cells
Publication Date：2024-06-09
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1-7 )