GZMA--a prognostic biomarker for IDH mutant glioma and a correlator with immune infiltration

QIANG Yilong; MA Chenyu; ZHANG Yanyu; LIN Wei

Abstract：Objective To explore the prognostic value of Granzyme A(GZMA)in gliomas and its correlation with immune cell infiltration.Methods Differential gene expressions correlative with the two sets of data from low-grade glioma(LGG,WHO GradeⅡ,IDH Mutant)and high-grade glioma(HGG,WHO Grade Ⅳ,IDH Mutant)in the glioma transcriptome dataset of CGGA database were analyzed by bioinformatics tool(R language).The GO/KEGG enrichment analysis of the upregulated gene set was carried out by using R software package.The relationship between GZMA expression and Glioma immune infiltration was analyzed through network platforms including TIMER and GEPIA as well as TCGA data sets.Simultaneously,effects of GZMA on the survival span of the neuroglioma patients were evaluated.Results The expression level of GZMA was upregulated in IDH-mutant high-grade gliomas.Outcomes of gene set enrichment analysis showed that GZMA was involved in several top and significantly enriched GO categories.Compared to normal brain tissue,mRNA expression levels of GZMA in all the glioma samples were upregulated and relevant to both prognosis and classification of glioma patients.Kaplan Meier(K-M)survival curve analysis indicated that patients with high GZMA expression had shorter overall survival(OS)time than those with low GZMA expression.Moreover,the expression level of GZMA might affect the immune infiltration level in gliomas.The expression level of GZMA in GBM was positively correlated with expression levels of both B cells and dendritic cells,while negatively associated with expression levels of CD8+T cells and CD4+T cells.In contrast to GBM,the expression level of GZMA was positively correlated with infiltration levels of various immune cells.Conclusion GZMA can function as a potential biomarker in the judgment of prognosis and immune cell invasion in glioma.