The role of ALDH2 in neuronal injury induced by high glucose

WANG Xiaoliang; XIONG Jian

Abstract：Objective To test the role of ALDH2 in diabetic encephalopathy.Methods HT-22 neuron model cultured in high glucose in vitro and Alda-1,an agonist of ALDH2,were utilized to explore the role of ALDH2 in neurons cultured in high glucose in vitro.Firstly,the expression rule of ALDH2 at different concentrations and processing times was explored.Secondly,Alda-1 was used to treat HT-22 neuronal cells cultured with high glucose,and neuronal injury was also evaluated by TUNEL,Hoechst-PI staining,and detection of LDH expression.Finally,ROS,MDA,SOD,GSH-Px,IL-1β,and IL-18 were detected to evaluate the oxidative stress and neuroinflammation.Results As high glucose could suppress the expression of ALDH2 in a manner dependent on both concentration and time,Alda-1 was employed to treat HT-22 cells cultured in high glucose.Through TUNEL staining and LDH detecting,Alda-1 could be found to inhibit apoptosis.Hoechst PI staining showed that Alda-1 could reduce Hoechst+/PI+cells,while RT-qPCR indicated that Alda-1 could downregulate the expression level of RIP and MLKL(markers of necroptosis).Ultimately,detection of ROS,MDA,SOD,GSH-Px,IL-1β and IL-18 manifested that Alda-1 could inhibit neuronal oxidative stress and neuroinflammation.Conclusion ALDH2 could suppress programmed death,oxidative stress and neuroinflammation to protect neurons away from high glucose.