Highly expressed specific genes of immune and microglia/macrophage cells in glioblastoma possess the feature of mesenchymal subtype
LV Rui
JI Ang
XUE Xiaoyan
ZHANG Xiang
MAO Xinggang
Abstract：Objective To investigate the specific gene sets highly expressed in immune cells,microglia/macrophages,in glioblastoma(GBM),in order to deepen the understanding of both structure and function of the immune microenvironment in glioblastoma.Methods Molecular expression levels in different types of cells in normal and GBM tissue were analyzed respectively,and genes specifically high expressed(MM-Genes)in immune and/or microglia/macrophages cells(MM-Cells)were acquired.Both expression levels of MM-Genes in different grades and molecular subtypes of gliomas,and impact of MM-Genes on patient prognosis,were further investigated.Results A series of highly reliable MM-Genes,including CD74,HLA-DRB5,CCL3,NFAM1,DOCK8,LTC4S,GPR183,CCL4,SLAMF8,NCKAP1L,CD14,CD68 and PLCB2 were obtained.The majority of MM-Genes were highly expressed in gliomas.In addition,the higher the grade of GBM was,the more expression of MM-Genes higher.Furthermore,MM-Genes were also highly expressed in the mesenchymal subtype of GBM.Secondly,most individual genes of MM-Genes,as well as the integrative score of MM-Genes(MM-GScore),were both associated with prognosis of patient.Patients with upregulated MM-GScore had poor prognosis.Conclusion MM-Genes were highly correlated with grade of glioma and mesenchymal subtype of GBM.High expression of MM-Genes revealed poor prognosis.It disclosed the relationship between immune regulation,glioma grade and molecular subtype.
Keywords：GlioblastomaGliomaImmunotherapyMacrophagesMicrogliaImmune regulationMesenchymal subtype
Publication Date：2024-02-09
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 38-45 )