The expression and significance of PINK1-mediated mitochondrial autophagy in mouse cortical neurons after oxygen-glucose deprivation
WANG Yuanxin
ZHANG Lei
HEI Yue
YU Yang
YUE Kangyi
WU Xiuquan
JIANG Xiaofan
Abstract：Objective The temporal and spatial expression changes of mitochondrial autophagy related protein-PTEN induced putative kinase 1 (PINK1) at different time points after oxygen-glucose deprivation (OGD) in mouse cortical neurons were investigated.Methods Primary cortical neurons derived from C57BL/6J mouse were cultured for 7 d and then different time points (2 h/4 h/6 h/8 h) after OGD were used as set points.Morphological change was detected by inverted microscope;cell viability was detected by MTT;temporal variations of LC3,PINK1,Parkin was detected by Western blot;spatial variations of the expression of PINK1 was detected by immunofluorescence.Results The cell viability was impaired by OGD,and aggravated as the duration of OGD increased.The expression of LC3-Ⅱ and PINK1 peaked at 4 h after OGD (P ＜0.05),and the expression was gradually decreased since then.Parkin was decreased meanwhile (P ＜ 0.05).PINK1 was evenly expressed in cytoplasm and the intensity of immunofluorescence was temporally increased after OGD and was gradually decreased after that.Conclusion Different periods of OGD treatment induce the neuronal damage at different levels,and subsequently activate the PINK1-mediated mitochondrial autophagy,which may play a role in the pathophysiological process of cerebral ischemia via mitophagy.
Keywords：PTEN-induced putative kinase protein 1Microtubule related protein-1 light chain-3Oxygen-glucose deprivationNeuronsMitophagy
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 143-146 )