The role of LPS in inflammation after traumatic brain injury

FAN Youdao; YIN Haibin; YANG Yong; ZHANG Wangming

Abstract：Objective The role of LPS in inflammation after traumatic brain injury was analyzed.Methods A total of 36 cases of traumatic brain injury patients treated in our hospital between September 2010 to September 2014 were studied retrospectively.All the patients were divided into mild,moderate,and severe traumatic brain injury groups according to Glasgow coma scale (GCS),with 12 patients in each group.And 12 healthy cases who took medical examination in the hospital were selected as control group.All patients were collected 3 mL peripheral venous blood at 1 d,2 d,3 d,5 d,7 d,and 14 d after injury and serum was isolated.Serum TNF-α and IL-6 levels were detected by ELISA.Serum NO levels were detected by GREISS.Results TNF-α and IL-6 levels were significantly different between severe,moderate injury group and control group at 1 d,2 d,3 d,5 d,and 7 d post-injury (P ＜0.05).TNF-α and IL-6 levels in mild injury group were significantly different from control group at 1 d,2 d,3 d,5 d,and 7 d post-injury (P ＜0.05).Serum TNF-α and IL-6 levels at 14 d post-injury between mild injury group and control group were not significantly different (P ＞0.05).Serum NO concentration of severe injury group at 1 d,2 d,3 d,5 d,7 d,and 14 d was significantly different from that of the control group (P ＜0.05).There was significant difference in serum NO concentration at 1 d,2 d,3 d,5 d,and 7 d post-injury between mild injury,moderate injury and control group (P ＜0.05).But serum NO concentration in mild injury and moderate injury group at 14 d post-injury was not significantly different (P ＞0.05).Conclusion LPS plays a catalytic role in the inflammatory response after traumatic brain injury.