The synergistic effects and mechanism of astragaloside Ⅳ and hydroxysafflor yellow A against cerebral ischemia reperfusion
XU Hang
DING Yi
LIU Wenxing
LIU Tianlong
WEN Aidong
Abstract：Objective The effects and mechanism of astragaloside Ⅳ (AST-Ⅳ) and hydroxysafflor yellow A (HSYA) in combination against cerebral ischemia reperfusion in rats were studied.Methods One hundred Sprague Dawley rats were randomly divided into sham group,model group,AST-Ⅳ group,HSYA group and AST-Ⅳ + HSYA group.The model of middle cerebral artery occlusion (MCAO) was established by thread ligation.Neurological system symptoms were evaluated at 72 h after ischemia repeffusion.The histopathological changes of brain tissues were stained with hematoxylin and eosin (HE) and the platelet endothelial cell adhesion molecule-1 (CD31) expression was detected via immunohistochemistry.The markers of oxidation and inflammation including superoxide dismutase (SOD),malondialdehyde (MDA),tumor necrosis factor-α (TNF-α) and interleulin-1β (IL-1β) were examined.Results The symptoms of neurological injury in treatment groups were improved significantly compared with those in model group.The expressions of CD31 were increased,the activities of SOD and glutathione peroxidase (GSH-Px) were improved,and the levels of TNF-cα and IL-1β were decreased.Moreover,the most remarkable effect of treatment occurred in AST-Ⅳ and HSYA combination group.Conclusion AST-Ⅳ and HSYA in combination can exhibit obvious synergistic protective effects on ischemia reperfusion injury in rats,which is related to antiinflammation,anti-oxidation and angiogenesis pathway.
Keywords：Cerebral ischemia reperfusionAstragaloside ⅣHydroxysafflor yellow A
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 427-431 )