Expression and biological function of LncRNA MEG3 in glioma

ZHU Feng; ZHANG Peng; SONG Jianrong; LV Xinwen; ZHOU Xiaolong; CAI Ke; ZHANG Chao; HE Bei; LIANG Yuan

Abstract：Objective The expression and biological functions of LncRNA maternally expressed gene 3 (MEG3) in glioma were investigated.Methods Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect lncRNA MEG3 expression of 116 cases of glioma and adjacent normal brain tissues.And MTS [3-(4,5-diethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-etrazolium,inner salt] assay,flow cytometry and Transwell assay were further used to analyze the proliferation,apoptosis,and migration of MEG3 overexpression.Results LncRNA MEG3 expression was significantly decreased in the glioma compared with the normal tissues (4.85 ±0.56 vs 9.18 ±0.45;t =1.67,P =0.0012 ＜0.05);overexpression of MEG3 inhibited the cell proliferation (62.0％ ±3.8％ vs 84.0％ ±4.6％;t =1.54,P =0.0145 ＜0.05),promoted the cell apoptosis (19.4％ ± ±3.2％ vs 5.5％ ± 1.2％;t =2.35,P =0.0013 ＜0.05) and inhibited the cell migration (55.6％ ± 5.8％ vs 100％ ± 0;t =1.48,P=0.0021 ＜0.05) in U87 human glioma cell line.Conclusion LncRNA MEG3 is lowly expressed and indicates the inhibition of proliferation and migration and promotion of apoptosis in glioma and implicates the potential application of MEG3 in glioma therapy.