In vitro study of antitumor effect of levetiracetam combined with temozolomide in glioma cell lines
WU Qingwu
ZHANG Zongping
WANG Jing
CHEN Furong
ZHAO Yiying
QU Yue
CHEN Zhongping
Abstract：Objective The antitumor effect of levetiracetam (LEV) combined with temozolomide (TMZ) was investigated in glioma cell lines.Methods The cell proliferation with cell counting kit-8 (CCK-8) assay was performed in two O6-methylguanine DNA methyltransferase (MGMT) positive cell lines (T98G and U138) and two other MGMT negative cell lines (SKMG-4 and U87).The cells were treated with LEV and TMZ alone or in combination.Results LEV inhibited the proliferation of glioma cell hnes but did not reach the therapeutic effect,especially in U87.The 50％ inhibitory concentration (IC50) of TMZ was significantly decreased in cell lines (T98G,U138 and SKMG-4,P ＜ 0.05) which were treated with TMZ and LEV with no significant changes in U87 (P ＞ 0.05).Conclusion LEV may inhibit the proliferation of glioma cell lines (both MGMT-positive and MGMT-negative) and also sensitizes the cell lines (T98G,U138 and SKMG-4,but not U87) to TMZ.
Keywords：GliomaLevetiracetamTemozolomideMGMT
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 217-220 )