The association between hippocampal sphingosine-1-phosphate receptor 1 expression and neural stem cells proliferation following traumatic brain injury
YE Yuqin
YANG Yongxiang
SU Xinhong
HE Jun
CHEN Xiaoyan
HE Xiaosheng
Abstract：Objective The potential association between hippocampal sphingosine-1-phosphate receptor 1(S1PR1) expression and neural stem cells (NSCs) proliferation in a rat model of traumatic brain injury (TBI) was studied.Methods Ninety-six rats were randomly divided into TBI 1 d group,3 d group,7 d group,14 d group,21d group,28 d group (10 in each),and six control groups (6 in each).TBI model was induced by a controlled cortical injury device.NSCs were double-labeled by thymidine analog 5-Bromo-2-deoxyUridine (BrdU) and sex determining region Y-box 2 (Sox2) with immunofluorescence staining.The level of S1PR1 protein in hippocampus was detected by Western Blot at scheduled time-points after TBI.Results NSCs in hippocampus was activated at 1 d after TBI,reached the peak at 7 d,was followed by a decrease from 7 d to 28 d when maintained a higher level compared to control group (P ＜0.05).Hippocampal S1PR1 protein was increased from 1 d post trauma,and peaked around 7 d,decreased at 14 d,21 d,and 28 d to a lower level but still higher than that of control group (P ＜0.05).Conclusion The S1PR1 expression varies in a similar temporal pattern with NSCs proliferation in hippocampus,indicating that S1PR1 may be required for the neurogenesis after TBI.
Keywords：Traumatic brain injurySphingosine-1-phosphate receptor 1Neural stem cellsHippocampus
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 110-114 )