Effect of isoliquiritigenin on LDH and SOD activity in PC12 cells before and after hypoxia

JI Wei; LI Changdong; LI Zhiyun; SUN Jianjun; FU Yajie

Abstract：Objective Effect of isoliquiritigenin (ISL) on lactate dehydrogenase (LDH) and superoxide dismutase (SOD) in hypoxia-induced nerve cell damage was discussed.Methods PC12 cells were cultured in vitro to establish hypoxic injury model.The cells were divided into C (control group), H (model group, namely the hypoxia group ), H1 ( isoliquiritigenin low dose group ), H2 ( isoliquiritigenin middle dose group ), H3 (isoliquiritigenin high dose group).H1, H2, H3 with corresponding concentration of hormone and H were put in hypoxic environment for culture.Morphology of PC12 were observed under microscope.Lactate dehydrogenase and SOD in the supernatant lactate were determined.Results LDH in PC12 cells after hypoxia treatment was increased significantly, while SOD was significantly decreased.After adding isoliquiritigenin ( ISL ), LDH in the cell supernatant lactate was significantly decreased, but SOD was increased.Conclusion Hypoxia inhibits the proliferation of PC12 cells to some extent, by reducing SOD activity and increasing lactate LDH leakage .ISL can reverse the inhibition of hypoxia on PC12 cells.It may protect the cells by improving the SOD activity in culture medium, preventing lipid peroxidation, maintaining membrane stability, and reducing LDH release.