Transcriptional factor ZNF580 promotes eNOS expression in vascular endothelial cells
ZHANG Lei
LUO Yuyu
CHANG Pengfei
SUN Shizhong
Abstract：Objective The effect of the novel zinc-finger nuclear transcription factor ( ZNF580 ) on the expression of endothelial nitric oxide synthase ( eNOS) in vascular endothelial cells is discussed.Methods The changes of gene expression profiling of ZNF580-overexpressed EA.hy926 cells were detected by gene microarray.The ZNF580 and eNOS expressions of the EA.hy926 endothelial cells which transfected with Lenti-GFP-ZNF580 and Lenti-RNAi-ZNF580 were detected by the real-time polymerase chain reaction (RT-PCR) and Western bolt.Results The results of gene chip showed that there were many gene expression changes in ZNF580-overexpressed EA.hy926 cells. The expression of eNOS was markedly up-regulated.The mRNA and protein expressions of ZNF580 and eNOS were increased in EA.hy926 cells transfected with Lenti-GFP-ZNF580.But the eNOS and ZNF580 expressions were down-regulated in EA.hy926 cells transfected with Lenti-RNAi-ZNF580.Conclusion Transcriptional factor ZNF580 promotes the expression of eNOS in vascular endothelial cells.
Keywords：ZNF580eNOSCerebral angiospasm
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 302-306 )