Low frequency stimulation of pedunculopontine affects neurotransmitter level of ventrolateral thalamic nucleus in Parkinsonian rats
ZHANG Wangming
WEN Peng
LI Min
XIAO Hu
ZHOU Ming
WANG Qiang
XIE Zhengyuan
Abstract：Objective The effects of low frequency stimulation of pedunculopontine nucleus ( PPN ) on neurotransmitter of ventrolateral thalamic nucleu (VL) in unilateral 6-hydroxy dopamine (6-OHDA)-lesion rats are investigated.Methods Twenty-seven male SD rats were randomly assigned to sham-lesion group A (n=8), lesion group B (n=9), and lesion+electrodes group C (n=10).After stereotactic surgery, these samples were collected by microdialysis.The neurotransmitter in every sample was detected by high performance liquid chromatography and mass spectrometry (LC-MS).Results The 6-OHDA lesion resulted in a significant increase of the level of the glutamic acid (Glu) andγ-aminobutyric acid (γ-GABA) in VL, but a little decrease of acetylcholine (Ach) level. During PPN stimulation phase, the Glu concentration in VL was notably improved and the Ach concentration was mildly improved, but that of γ-GABA did not change.Conclusion Low frequency stimulation of PPN could rebalance the Glu and Ach level in VL nucleu of PD rats, which may be associated with its improving posture instability and gait difficulty.
Keywords：Parkinsonˊs diseaseDeep brain stimulationPedunculopontine nucleusNeurotransmitterRats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 221-224 )