The expression and significance of CPG15 in rat model of diffuse axonal injury

doi:10.3969/j.issn.1671-2897.2008.03.002

Abstract：Objective To investigate the expression and significance of CPGl5 (candidate plasticity related gene 15) in frontal brain cortex of rat model of diffuse axonal injury (DAI) at different posttraumatic time point. Methods The 54 male SD rats were divided randomly into normal control group (n=18) and DAI group (n=36). The DAI group was subdivided into 1d、4d、7d、10d、14d、21d group with 6 in each. The DAI model was produced by lateral head rotation device. The expression of CPGl5 in the frontal cortex in rat brain was investigated by immnohistochemical method and its change was also analyzed. The occurrence of yellow-brown particles was considered as "positive", and lack of the stain as "negative". Results No expression was found in the frontal cortex in normal rat brain. The expression of CPGl5 existed in small amount in the frontal brain cortex in the rat 1 day post DAI, and at this time the number of neurons was significantly decreased. The expression became stronger gradually at 4,7 and 10 day, and reahed to the peak at 14 day. It maintained at higher level until 21 day post DAI. Meanwhile, the neurons increased in number with the time internal post DAI. Conclusion The expression of CPI15 and the number of neurons were much associated positively with each other post DAI. The underlying mechanism and significance were discussed.