Prestroke DNA immunization against neurite growth inhibitors enhanced neurite regeneration following focal cerebral ischemia
ZHU Xing-bao
Jasmine Lee
Jill Wong
Wan Loo Tan
WANG Ting-hua
FENG Zhong-tang
Xiao Zhicheng
Lvan Ng
Abstract：Objective To elucidate the effect of prestroke DNA immunization against neurite growth inhibitors (NGIs) on neurite regeneration following permanent focal cerebral ischemia. Methods DNA immunization was arried out by intramuscular injection of pcDNA3. 1 ( + )-neurite growth inhibitors (pcDNA-NGIs) into the tibial muscle once a week for 6 weeks. Left focal cerebral ischemia was permanently induced through the occlusion of the middle artery (MCAO) after pcDNA-NGIs-induced auto-antibodies in sera were detected. The newly generated axons of corticorubral projection were traced by stereotaxic guided injection of biotinylated dextran amine (BDA). Results Prestroke DNA immunization against NGIs led to an increased compensatory newly growth of axons from corticorubral projection following focal cerebral ischemia. Conclusion Prestroke DNA immunization with pcDNA-NGIs is beneficial to neurite regeneration following permanent focal cerebral ischemia.
Keywords：Neurite growth inhibitorsDNA immunizationFocal cerebral ischemiaNeurite regeneration
Publication Date：2008-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 21-24 )