Effect of DNA immunization against neurite growth inhibitors on the central nervous systm in rats
ZHU Xingbao
Jasmine Lee
Jill Wong
Wan Loo Tan
FENG Zhongtang
XIAO Zhicheng
Ivan Ng
Abstract：Objective To determine if rats risk their central nervous system for DNA immunization against neurite growth inhibitors(NGIs).Methods Adult male Sprague-Dawley rats were treated with pcDNA3.1(+)-neurite growth inhibitors(pcDNA-NGIs),a DNA vaccine against NGIs.During the pcDNA-NGIs immunization period,a modified experimental autoimmune encephalomyelitis scoring system including pathological scores and neurological scores,together with a series of sensorimotor and cognitive tasks,was performed.Results The neurological scores showed that rats immunized with pcDNA-NGIs did not have any experimental autoimmune eneephaloemyelitis(EAE)symptom and the pathological scores showed that no inflammation,demyelination and axonal loss in the brains and spinal cords of these rats.Consequently,composite neurological scores,adhesive tape removal task and open field test indicated that pcDNA-NGIs immunized rats did not have any sensorimotor deficit.Also,passive avoidance task and active avoidance task showed that no deficit of learning and memory presented to these rats.Conclusion Significant adverse effects spare the central nervous system of pcDNA-NGIs immunized rats.
Keywords：DNA vaccineNeurite growth inhibitorsExperimental autoimmune encephalomyelitisSensorimotor deficitCognitive behavior
Publication Date：2007-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 214-218 )