Expression of cyclin D1/D2/D3 in the tumors of Wistar C6 brain glioma models
JI Xituan
ZHANG Xiang
FEI Zhou
ZHANG Jianning
LIU Weiping
JIANG Xiaofan
SONG Shaojun
SONG Lei
ZHAO Ming
Abstract：Objective This experiment was designed to detect the expression of cyclin D1/D2/D3 in the tumor tissues of rat glioma models. Methods The C6 cells cultured in vitro were stereotaxically implanted into the left caudate nucleus of Wistar. The concentration of C6 cells was 5 × 109/L ( the injecting volume was 60 μl for each rat). After C6 inoculation, the living and survival of animals were observed.Tumor was confirmed with HE and immunohistochemistry staining of GFAP. Cyclin D1/D2/D3 were detected by immunohistochemistry. Results The study demonstrated the expression of cyclin D in glioma tissues. Immunohistochemical reaction of cyclin D1/D3 was confined to the nucleus, while the expression of cyclin D2 was mainly in cytoplasm. There was slight difference of expression of all kinds of cyclin D among three study groups. Conclusion The data suggest that amplification and increased expression of cyclin D family contribute to the loss of cell cycle control in a small fraction of Wistar C6 gliomas. The overexpression of cyclin D family is negatively related with survival time of the rats with tumors.
Keywords：Gliomacyclin D1/D2/D3Rat
Publication Date：2007-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 125-128 )