Experiment study on bioluminescent signal in orthotopic and heterotopic brain tumors in nude mice
BU Xingyao
ZHANG Xiang
Walter E. Laug
Abstract：Objective To investigate the bioluminescent signal in orthotopic and heterotopic brain tumors in nude mice for noninvasive monitoring on progression of brain tumor in vivo.Methods The human brain tumor cell line U87MG cells were transduced by using the SMPU-R-MND-luc vector then a clone with high luciferase activity was cloned and used for in vivo experiments. Tumor cells were implanted into the brains and flanks of the animals, and whole body images revealing tumor location were obtained. Tumor burden was monitored over time by quantity of photon emission.Results Orthotopic brain tumor and heterotopic flank tumor models were established by implanting constitutively expressing luciferase tumor cells in the brains and flanks of the animals. The magnitude of bioluminescence from firefly luciferase measured in vivo correlated with the time after injection of luciferin, indicating that the time of post-injection signal quantification was of special importance. Furthermore, the time courses in the heterotopic and orthotopic tumors were observed significantly different. However, kinetic studies performed on the 4 th and 14 th day post-cell implantation revealed no significant differences in orthotopic tumors.Conclusion This model system with in vivo bioluminescence imaging is an excellent experimental animal model in research for noninvasive monitoring on progression of brain tumor in vivo.
Keywords：LuciferaseNoninvasive monitoring ImagingBrain tumorOrthotopicHeterotopicNude mice
Publication Date：2006-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 224-228 )