Experimental study on the establishment for orthotopic implantation model of human glioma cells in nude mice
BU Xingyao
ZHANG Xiang
Walter E. Laug
Abstract：Objective To explore the establishment for orthotopic implantation model of human glioma cells in nude mice.Methods The human brain glioma cell line U87 MG was cultured in vitro, and cells (resuspended in serum-free RPMI 1640 medium at 108/ml) were inoculated by injection with stereotactic guidance into the frontal white matter of nude mice. General observation was conducted after injection. The animals were sacrified at intervals from 1 h to 63 d after implantation and the brain were examined by pathology and immunohistochemistry and tumor size was measured.Results Optimal injection rate was found to be 0.05 μl/min = 1 μl/20 min, a volume of 1 μl containing 105 cells and injection was done over 20 min. Tumor cells formed a nice sphere in the caudate putamen, away from ventricle as well as brain surface with no noticeable reflux into the needle track. Gliomas were formed in 100% nude mice. And the growth of tumors was stable in the regions of intracerebral environment in which tumors showed the morphological features of human glioma.Conclusion The method allows the highly accurate and reproducible introduction of a given number of cells into a specific area of the nude mice brain. The tumor pathological characteristics mimic human brain glioma. The glioma model in nude mice is reliable animal model for the study of the tumorigenesis, biological characteristics and the evaluation of various treatment modalities.
Keywords：GliomaXenograftCell cultureAnimal modelNude mice
Publication Date：2006-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 100-105 )