Research on tumor-suppressive effect of HAC purified from C6 glioma cells

CHEN Zan; JIAN Fengzeng; WANG Yilong; LING Feng; DU Jianxin

doi:10.3969/j.issn.1671-2897.2006.01.007

Abstract：Objective To study the tumor-suppressive effect of purified heat shock protein-antigen complex (HAC) in SD rats immunized with HAC. Methods Immuno-affinity chromatography was used to purify HAC from C6 cells. Twenty rats were immunized with HAC to form the experimental group, and other 20 non-immunized rats served as the control group. One week after immunization, C6 cells were inoculated into the brains of the rats by stereotaxic method. Two weeks later, peripheral venous blood was drawn, total lymphocyte count was done, and the rates of CD3 +/CD4 + , CD3 +/CD8 + T lymphocytes were measured by flow cytometry. Four weeks after inoculation,the symptoms manifested by the animals were observed and their survival rates were estimated. All the rats were killed and their brain tissues were sent for histopathologic and immunohistochemical examinations. Results The number of total peripheral blood lymphocytes and CD3 +/CD4 + , CD3 +/CD8 + T lymphocytes of the experimental group rats were significantly higher than those in the control group rats (P ＜0. 01 ). The quantity of CD3 + and CD4+T lymphocytes that infiltrated into the tumor tissues of the experimental group rats was significantly larger than that of the control group rats (P ＜0. 01 ). There was no significant difference in CD8+ T lymphocyte infiltration between these two groups. The ratio of CD4 +/CD8 + T lymphocytes of the experimental group rats was significantly higher than that of the control group rats (P ＜0.01 ). The survival rate of the experimental group rats was significantly higher than that of the control group rats (P ＜ 0. 05). Conclusion Rats immunized with HAC purified from C6 cells develop immuno-resistance against C6 glioma cells, and their survival rates are improved.