In vitro experiment research of the anti-glioma activity of the glioma vaccine made from dendritic cell
JIAO Baohua
LI Chunhui
ZHANG Qingjiu
BAI Baozhong
GENG Shaomei
Abstract：Objective To discuss the mechanism of the anti-glioma activity of the tumor vaccine made from the dendritic cell in vitro.Methods Culture dendritic cells of the rat, extract the glioma antigen by freeze-melt method and then get the vaccine by pulsing the denderitic cells with the glioma antigen. The experiment rats were divided into 4 groups. The vaccine, dendritic cells and 0.9% (g/ml) chlorine sodium saline were perfused to the rats. At the prearranged time, the spleen of the rats was taken out and was examined the anti-tumor activity of the T lymphatic cell of the spleen by methyl thiazolyl tetrazolium (MTT) method.Results The anti-tumor activity of the lymphocyte of the rats which was injected with glioma vaccine once was lower than that of the rats which were injected for 3 times. There was no statistic difference between the kill activity to the glioma cells of the lymphatic cells of the rats injected with dendritic cells cultured 8 days and the lymphatic cells of the rats injected with 0.9% (g/ml) chlorine sodium saline.Conclusion Glioma vaccine made from dendritic cells can stimulate the immune activity to kill the glioma cells obviously.
Keywords：Dendritic cellTumor vaccineGliomaImmunity therapy
Publication Date：2005-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 486-491 )