Protective role of heme oxygenase-2 gene deletion in oxidative stress-induced brain injury
QU Yan
ZHANG Xiang
CHEN Jing
MA Xin
Raymond F.Regan
Abstract：Objective To investgate heme oxygenase-2 gene deletion decreasing oxidative stress injury induced by heme in vivo.Methods Stereotactic injection of 6 μ l of a sterile hemin (8 μmol/L) into the striatum of wild type and HO-2 knock out mice. Cell viability, protein oxidation, and lipid oxidation of the striatum were determined after 72 h injection. Western blot analysis was applied for heme oxygenase-1 (HO-1) measurement.Results Protein oxidation and lipid oxidation decreased significantly in HO-2 knockout mice at 72 h compared with that of wild-type mice. Striatal cell viability increased significantly in knockout mice at 72 h compared with that of wild-type mice. HO-1 expression at baseline and 72 h after hemin injection was also similar to that of wild-type mice.Conclusion These results suggest that HO-2 gene deletion protects neurons from heme mediated oxidative stress injury. Selective inhibition of neuronal HO-2 may have a beneficial effect on oxidative stress-induced neuronal injury.
Keywords：Heme oxygenaseGene deletionOxidative stressBrain injury
Publication Date：2005-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 197-203 )