Correlation of serum microRNA-760 and cytoplasmic polyadenylation element binding protein 2 expression with clinicopathological features and prognosis in children with acute lymphoblastic leukemia

Bai Taomin; Liu Hong; Wang Xia

doi:10.3760/j.issn.1673-4777.2026.05.016

Abstract：Objective To investigate the correlation of serum microRNA-760(miR-760)and cytoplasmic polyadenylation element binding protein 2(CPEB2)expression with clinicopathological features and prognosis in children with acute lymphoblastic leukemia(ALL).Methods A total of 205 children with ALL admitted to Shaanxi Provincial People's Hospital from January 2020 to June 2022 were prospectively enrolled as the ALL group,and 103 healthy children during the same period were selected as the control group.The serum expression levels of miR-760 and CPEB2 mRNA were detected.The binding site between miR-760 and CPEB2 was predicted through an online database.Pearson correlation analysis was used to analyze the correlation between serum miR-760 and CPEB2 mRNA expression in children with ALL,and their relationship with clinicopathological features was also analyzed.The progression-free survival rate was compared among children with different serum expression levels of miR-760 and CPEB2 mRNA.Multivariate Cox regression analysis and receiver operating characteristic(ROC)curve were used to analyze the influencing factors for the prognosis of children with ALL and the predictive efficacy of serum miR-760 and CPEB2 mRNA expression for prognosis.Results The serum expression level of miR-760 in the ALL group was lower than that in the control group[(0.64±0.11)vs(1.01±0.17)],and the expression level of CPEB2 mRNA was higher than that in the control group[(1.33±0.30)vs(0.83±0.14)](all P＜0.001).A binding site of miR-760 was found at positions 2855-2861 of the 3'untranslated region of CPEB2.Serum miR-760 expression was negatively correlated with CPEB2 mRNA expression in children with ALL(r=-0.655,P＜0.001).There were statistically significant differences in the serum expression levels of miR-760 and CPEB2 mRNA among ALL children with different risk stratifications(all P＜0.001).The 3-year event-free survival rate of ALL children in the miR-760 high expression group was higher than that in the miR-760 low expression group,and the 3-year event-free survival rate in the CPEB2 mRNA high expression group was lower than that in the CPEB2 mRNA low expression group(all P＜0.05).Mixed lineage leukemia gene rearrangement,high-risk stratification and CPEB2 mRNA≥1.33 were independent risk factors for poor prognosis in children with ALL,while miR-760 ≥0.64 was an independent protective factor(all P＜0.05).The areas under the curve of serum miR-760,CPEB2 mRNA and their combination in predicting poor prognosis of children with ALL were 0.796,0.790 and 0.896,respectively.The combined detection was superior to the single detection of serum miR-760 or CPEB2 mRNA in predictive efficacy(all P＜0.05).Conclusion Serum miR-760 low expression and CPEB2 mRNA high expression are associated with adverse clinicopathological features and poor prognosis in children with ALL,and the combined detection of serum miR-760 and CPEB2 expression has a high predictive efficacy for the prognosis of these children.