Study on the mechanism of compound hormone preparation improving ovarian reserve and endometrial recep-tivity in mice with premature ovarian failure combined with chronic psychological stress
Aziguli Alimujiang
Maimaiti Yisireyili
Li Siqi
Wang Yongkang
Lu Ping
Han Lili
Abstract：Objective To explore the mechanism of estradiol tablets/estradiol and dydrogesterone tablets(femoston)regulating hypothalamic-pituitary-ovarian(HPO)axis to improve ovarian reserve and endometrial receptivity in premature ovarian failure mice with chronic psychological stress.Methods Thirty female Kunming mice aged 8 weeks were completely randomly divided into control group,model group and treatment group,with 10 mice in each group.The model of premature ovarian failure and chronic psychological stress was established in the model group and the treatment group.The treatment group was given femoston by gavage for 4 weeks,and the control group and the model group were given normal saline by gavage.The levels of serum sex hormones,pathological changes of ovarian and uterine tissues,the content of leukemia inhibitory factor(LIF)and the expression of HPO axis related genes in endometrial tissues were detected and compared.Results Compared with the control group,the levels of serum follicle-stimulating hormone(FSH)and luteinizing hormone(LH)in the model group were increased,and the level of estradiol was decreased.The number of ovarian antral follicles decreased and the stromal cells proliferated.The endometrium became thinner,the number of glands decreased and irregular,and the level of LIF decreased.The gene expressions of FSHβ and LHβ were increased,while the gene expressions of gonadotropin-releasing hormone(GnRH),estrogen receptor α(ERα)and estrogen receptor β(ERβ)were decreased(all P＜0.05).Compared with the model group,the levels of FSH and LH in the treatment group were decreased,and the level of estradiol was increased.The number of primary ovarian follicles was increased,and the proliferation of stromal cells was alleviated.The endometrial thickness,glandular structure and LIF level were increased.The gene expression of FSHβ and LHβ decreased,while the gene expression of GnRH,ERα and ERβ increased(all P＜0.05).Conclusion Femoston can regulate HPO axis and improve ovarian reserve and endom-etrial receptivity in mice with premature ovarian failure combined with chronic psychological stress.
Keywords：Premature ovarian failureEstradiol tablets/estradiol and dydrogesterone tabletsChronic psychological stressOvarian reserveEndometrial receptivity
Publication Date：2025-11-18
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 1675-1679 )