Changes and clinical significance of peripheral blood endothelial progenitor cell microparticles and microRNA-126 expression in patients with acute ST-segment elevation myocardial infarction

Ailifeire Aihaiti; Liu Ke; Mailidan Maimaitiniyazi; Dilinigeer Alifujiang; Maidinaimu Abulizi; Kadierya Yibulayin; Muyesai Nijiati

doi:10.3760/j.issn.1673-4777.2025.08.001

Abstract：Objective To analyze the changes and clinical significance of peripheral blood endothelial progenitor cell microparticles(EPC-MPs)and microRNA-126(miR-126)expression in patients with acute ST-segment elevation myocardial infarction(STEMI)before and after ischemia-reperfusion.Methods Fifty patients with acute STEMI who received percutaneous coronary intervention(PCI)treatment at People's Hospital of Xinjiang Uygur Autonomous Region from December 2023 to November 2024 were selected.Based on whether myocardial ischemia-reperfusion injury(MIRI)occurred after PCI,they were divided into non-MIRI group and MIRI group.The clinical data of the patients were collected,and the peripheral blood of the patients was collected before PCI,immediately after PCI,and 4,12,24 h after PCI.Flow cytometry was used to identify and quantify EPC-MPs.Real-time fluorescent quantitative polymerase chain reaction was used to detect the expression level of miR-126 in microparticles.Logistic regression was used to analyze the correlation between relevant indicators and MIRI,and the receiver operating characteristic(ROC)curve was used to evaluate its diagnostic value for MIRI.Results During the study period,a total of 21 patients(42.0％)developed MIRI after PCI and were included in the MIRI group,and the remaining 29 patients were included in the non-MIRI group.The levels of EPC-MPs in peripheral blood of all patients showed a gradual increase trend after PCI.Between the two groups,the level of non-MIRI group was higher than that of MIRI group before 12 h after PCI,and lower than that of MIRI group after 12 h(all P＞0.05).The level of miR-126 in all patients increased gradually after PCI,reached the peak at 12 h,and then decreased.Between the two groups,the non-MIRI group was always higher than the MIRI group,and the difference was statistically significant at 12 h and 24 h after PCI(both P＜0.05).Multivariate Logistic regression analysis showed that the level of miR-126 at 24 h after PCI was independently associated with MIRI(odds ratio=0.20,95％confidence interval:0.04-0.87,P=0.031),and the area under the ROC curve was 0.810,with a sensitivity of 77.8％and a specificity of 76.2％.Conclusion The level of EPC-MPs in peripheral blood of patients with acute STEMI increases gradually after reperfusion,while the level of miR-126 increases first and then decreases.MiR-126 can be used as a potential diagnostic indicator for MIRI at 24 h after PCI.