Expression and clinical significance of long non-coding RNA SH3BP5-AS1 and microRNA-6838-5p in hepatocellular carcinoma patients

Zhang Tong; Ping Sheng; Li Zhenzhen; Ma Li

doi:10.3760/j.issn.1673-4777.2025.07.014

Abstract：Objective To investigate the expression of long non-coding RNA(lncRNA)SH3BP5-AS1 and microRNA(miR)-6838-5p in patients with hepatocellular carcinoma and their relationship with clinicopathological features and prognosis.Methods A total of 110 patients with hepatocellular carcinoma who underwent surgical treatment in The Second Affiliated Hospital of Xi'an Jiaotong University and XD Group Hospital from January 2018 to December 2020 were selected.The expressions of lncRNA SH3BP5-AS1 and miR-6838-5p in surgically resected cancer tissues and adjacent tissues were detected,and the survival of patients was followed up after discharge.The correlation between lncRNA SH3BP5-AS1 and miR-6838-5p in cancer tissues was analyzed,and Kaplan-Meier curve was drawn to compare the survival difference of hepatocellular carcinoma patients with different lncRNA SH3BP5-AS1 and miR-6838-5p expression.Univariate and multivariate Cox proportional hazards regression methods were used to analyze the prognostic factors of patients with hepatocellular carcinoma.Results The expression of lncRNA SH3BP5-AS1 in hepatocellular carcinoma tissues was higher than that in adjacent tissues,and the expression of miR-6838-5p was lower than that in adjacent tissues[(2.7±0.4)vs(1.5±0.3),(1.9±0.5)vs(3.1±0.7)](both P＜0.001).High expression of lncRNA SH3BP5-AS1 and low expression of miR-6838-5p were associated with poor differentiation,TNM stage Ⅲ,intrahepatic metastasis and vascular invasion(all P＜0.05).There was a negative correlation between lncRNA SH3BP5-AS1 and miR-6838-5p expression in hepatocellular carcinoma tissues(r=-0.524,P＜0.001).The 3-year overall survival rate of hepatocellular carcinoma patients with high expression of lncRNA SH3BP5-AS1 was lower than that of patients with low expression of lncRNA SH3BP5-AS1,and the 3-year overall survival rate of hepatocellular carcinoma patients with low expression of miR-6838-5p was lower than that of patients with high expression of miR-6838-5p(both P＜0.05).TNM stage Ⅲ,intrahepatic metastasis and high expression of lncRNA SH3BP5-AS1 were risk factors for poor prognosis of patients with hepatocellular carcinoma,and high expression of miR-6838-5p was a protective factor(all P＜0.05).Conclusion Up-regulation of lncRNA SH3BP5-AS1 expression and down-regulation of miR-6838-5p expression in hepatocellular carcinoma tissues are related to clinical malignant pathological characteristics and poor prognosis.