Correlation between the expression of circular RNA TTC3 and microRNA-135b-5p in peripheral blood and neurological deficits and prognosis in patients with acute cerebral infarction

Sun Jian; Sun Yan; Zhang Fan; Cheng Yi; Song Aixia

doi:10.3760/j.issn.1673-4777.2025.07.010

Abstract：Objective To investigate the expression of circular RNA TTC3(circTTC3)and microRNA-135b-5p(miR-135b-5p)in peripheral blood of patients with acute cerebral infarction(ACI),and to analyze their relationship with neurological deficits and prognosis.Methods A total of 149 ACI patients admitted to the First Affiliated Hospital of Hebei North University from January 2022 to June 2024 were selected as the observation group,and 120 healthy physical examination persons were selected as the control group.The expression of circTTC3 and miR-135b-5p in peripheral blood of the two groups was compared,and the correlation between circTTC3,miR-135b-5p and the National Institutes of Health Stroke Scale(NIHSS)score was analyzed.The related factors affecting the prognosis of ACI patients were analyzed,and the receiver operating characteristic curve was drawn to analyze the predictive value of circTTC3 and miR-135b-5p in the poor prognosis of ACI patients.Results The expression of circTTC3 in peripheral blood of the observation group was higher than that of the control group[(1.7±0.4)vs(1.1±0.3)],and the expression of miR-135b-5p was lower than that of the control group[(0.76±0.24)vs(1.09±0.36)](both P＜0.01).The expression of circTTC3 in peripheral blood of ACI patients in the mild group,moderate group and severe group gradually increased,and the expression of miR-135b-5p gradually decreased,and the differences between any two groups were statistically significant(all P＜0.01).In the observation group,the expression of circTTC3 in peripheral blood was positively correlated with the NIHSS score(r=0.318,P=0.006),and the expression of miR-135b-5p was negatively correlated with the NIHSS score(r=-0.295,P=0.009),and the expression of circTTC3 in peripheral blood was negatively correlated with the expression of miR-135b-5p(r=-0.272,P=0.011).Multivariate Logistic regression analysis showed that NIHSS score,low-density lipoprotein cholesterol,circTTC3 and miR-135b-5p were the independent risk factors for poor prognosis of ACI patients(all P＜0.05).The area under the curve of circTTC3 and miR-135b-5p in predicting the poor prognosis of ACI patients was 0.902,the 95％confidence interval was 0.829-0.953,the sensitivity and specificity were 93.42％and 71.51％,respectively.Conclusion The expression of circTTC3 is increased and the expression of miR-135b-5p is decreased in the peripheral blood of patients with acute cerebral infarction.Both of them are independent factors affecting the prognosis of patients with acute cerebral infarction,and their combined use has high clinical value in predicting the prognosis of patients with acute cerebral infarction.