Study on the effect and mechanism of echinacoside on the biological behavior and angiogenesis of ovarian cancer cells
Su Yuan
Jiao Yang
Xia Xinfeng
Zhang Na
Abstract：Objective To investigate the effect of echinoside on the proliferation,migration,invasion and angiogenesis of ovarian cancer cells and its possible mechanism.Methods Ovarian serous cancer cell line SKOV3 was used as the research subjects.Cell counting kit-8 was used to detect the effect of different concentrations of echinoside(0,6.25,12.5,25,50,100,200,400 μmol/L)on cell viability.SKOV3 cells were randomly divided into control group,low-dose echinacoside group(25 μmol/L),middle-dose echinacoside group(50 μmol/L),high-dose echinacoside group(100 μmol/L)and echinacoside high-dose+740Y-P(PI3K agonist,100 μmol/L)group.Colony formation assay,TUNEL staining and Transwell assay were used to detect cell proliferation,apoptosis,migration and invasion.Angiogenesis was detected by tube formation assay.Western blotting was used to detect the expressions of proliferation,apoptosis,invasion,angiogenesis and phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)pathway related proteins.Results The results of cell counting kit-8 showed that with the increase of echinoside concentration,the survival rate of SKOV3 cells decreased gradually,which were(100.0±5.5)％,(98.3±3.3)％,(93.3±3.4)％,(58.3±3.8)％,(42.4±4.6)％,(28.8±2.2)％,(19.4±3.4)％,(6.4±1.5)％,respectively,in a concentration dependent manner(P＜0.05).Compared with the control group,the number of SKOV3 cell colonies,migration,and invasive cells,the relative circular tubular structure formation ratio,the expression of cell proliferation antigen Ki-67,B-cell lymphoma 2(Bcl-2),N-cadherin,and vascular endothelial growth factor A(VEGFA),the phosphorylated(p)-PI3K/PI3K,p-AKT(S473)/AKT,p-AKT(T308)/AKT,and p-mTOR/mTOR ratios all gradually decreased in the low,medium,and high-dose echinacoside groups,while the apoptosis rate and expression of activate cysteine protease 3(Cleaved caspase 3),Bcl-2 related X protein(Bax),and E-cadherin gradually increased,showing a concentration-dependent effect,with statistically significant differences(all P＜0.05).Compared with the high-dose echinacoside group,the number of SKOV3 cell colonies,migration,and invasive cells,the relative circular tubular structure formation ratio,the expression of Ki-67,Bcl-2,N-cadherin,and VEGFA,the p-PI3K/PI3K,p-AKT(S473)/AKT,p-AKT(T308)/AKT,and p-mTOR/mTOR ratios all increased/rised in the echina-coside high-dose+740Y-P group,while the apoptosis rate and expression of Cleaved caspase 3,Bax,and E-cadherin all decreased,showing a statistically significant difference(all P＜0.05).Conclusion Echinoside can inhibit the proliferation,migration,invasion and angiogenesis of ovarian cancer cells,and its mechanism may be related to the inhibition of PI3K/AKT/mTOR signaling pathway.
Keywords：Ovarian cancerEchinacosideMalignant progressionPhosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway
Publication Date：2025-04-18
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：6( 571-576 )