Effect of aldolase A on the biology of bladder cancer cells and its mechanism

Li Jianwei; Cai Fangzhen; Liu Changjin

doi:10.3760/j.issn.1673-4777.2025.02.014

Abstract：Objective To investigate the effect and mechanism of aldolase A on the proliferation,migration,invasion and epithelial-mesenchymal transition(EMT)of bladder cancer cells.Methods The interference or overexpression plasmids of aldolase A were constructed by lentivirus infection and transfected into T24 human bladder transitional cell carcinoma(T24)cells.The cells were divided into control group,empty vector group,aldolase A interference group and aldolase A overexpression group.The expression of aldolase A in bladder cancer tissues and adjacent tissues was detected.The cell proliferation,migration and invasion were detected,and the expressions of EMT and Wnt/β-catenin signaling pathway related proteins were detected.Results The immuno-histochemical staining score and relative expression of aldolase A in bladder cancer tissues were higher than those in adjacent tissues[(33±7)vs(17±5),(63±6)％vs(22±4)％](both P＜0.001).After treatment with Cell Counting Kit-8 assay for 24,36 and 48 h,the absorbance values of T24 cells in aldolase A interference group were lower than those in the empty vector group,while the absorbance values of T24 cells in aldolase A overexpression group were higher than those in the empty vector group(all P＜0.05).The scratch healing rate of aldolase A interference group was lower than that of empty vector group,and the scratch healing rate of aldolase A overexpression group was higher than that of empty vector group(both P＜0.05).The number of invasive cells in the aldolase A interference group was less than that in the empty vector group,and the number of invasive cells in the aldolase A overexpression group was more than that in the empty vector group(both P＜0.05).The relative expression of E-cadherin in the aldolase A interference group was higher than that in the empty vector group,and the relative expression of N-cadherin,vimentin,β-catenin,cyclin D1,and c-Myc gene protein were lower than those in the empty vector group(all P＜0.05).The relative expression of E-cadherin in the aldolase A overexpression group was lower than that in the empty vector group,and the relative expression of N-cadherin,vimentin,β-catenin,cyclin D1,and c-Myc gene protein were higher than that in the empty vector group(all P＜0.05).Conclusion Aldolase A can promote the proliferation,migration,invasion and EMT of bladder cancer cells,which may be through the Wnt/β-catenin signaling pathway.